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| Strubbia, Sofia; Phan, My V. T.; Schaeffer, Julien; Koopmans, Marion; Cotten, Matthew; Le Guyader, Soizick. |
| This study aimed to optimize a method to identify human enteric viruses in sewage and stool samples using random primed next-generation sequencing. We tested three methods, two employed virus enrichment based on the binding properties of the viral capsid using pig-mucin capture or by selecting viral RNA prior to library preparation through a capture using the SureSelect target enrichment. The third method was based on a non-specific biophysical precipitation with polyethylene glycol. Full genomes of a number of common human enteric viruses including norovirus, rotavirus, husavirus, enterovirus and astrovirus were obtained. In stool samples full norovirus genome were detected as well as partial enterovirus genome. A variety of norovirus sequences was... |
| Tipo: Text |
Palavras-chave: Human enteric viruses; Norovirus; Sewage; Metagenomic; Virome. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00512/62332/66592.pdf |
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| Elamri, D; Aouni, M; Parnaudeau, Sylvain; Le Guyader, Soizick. |
| Aims: The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. Methods and Results: Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found... |
| Tipo: Text |
Palavras-chave: Shellfish; Reverse transcription PCR; Human enteric viruses. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1881.pdf |
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| Le Guyader, Soizick; Pommepuy, Monique; Atmar, Robert. |
| Human and animal fecal wastes and urine contain a large number of different viruses that can enter the environment through the discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. Viruses are present in shellfish in very low numbers. Nevertheless, they are present in sufficient quantities to pose a health risk as presented. This low level of contamination has made it necessary to develop highly sensitive viral extraction methods to ensure virus recovery from shellfish tissues. The most common route for accidental contamination is after heavy... |
| Tipo: Text |
Palavras-chave: Human enteric viruses; Shellfish; Sewage; Persistence; Flux. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00015/12606/15279.pdf |
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| Parnaudeau, Sylvain; Le Mennec, Cecile; Miura, Takayuki; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Human enteric viruses discharged in raw sewage are hightly resistant and may be detected in surface waters, coastal areas and shellfish. Whereas noroviruses, responsible for relatively benign acute gastroentritis, are the most common agents implicated in shellfish borne outbreaks, other human viruses which cause severe human diseases, such as hepatitis A virus, may also be transmitted. Environmental studies have demonstrated that virus concentrations detected in shellfish are higher than in surrounding waters. This concentration may explain the high prevalence of noroviruses detected in various shellfish species collected in different countries. If many questions remain regarding viral contamination of shellfish, the most important seems to be the... |
| Tipo: Text |
Palavras-chave: Shellfish; Human enteric viruses; Norovirus; Hepatitis A virus; Coquillage; Virus entérique humain; Norovirus; Virus de l'hépatite A. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00317/42849/42258.pdf |
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